UBE2Q2 (NP_775740.1, 1-375aa) full-length human protein.
The ubiquitination pathway is known to regulate critical control points in multiple cellular processes by targeting proteins for activation, degradation, or localization at specific intracellular sites. The pathway involves an enzymatic cascade in which the first step activates ubiquitin by forming a high energy thiolester intermediate to ubiquitin activating enzyme (E1) that is subsequently transferred to members of an E2 ubiquitin conjugating enzyme superfamily. The E2 ubiquitin conjugating 45 enzymes function together with a larger superfamily of E3 ubiquitin ligases to transfer activated ubiquitin to specific cellular proteins. The existence of E2 and E3 superfamilies reflects the specificity of each E2-E3 cognate pair for a specific group of cellular substrates. UBE2Q2 is a potentially unique member of the ubiquitin conjugating enzyme superfamily that includes at least 17 phenotypically different subfamilies, many of which contain isozymes. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
