Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 27kD band seen in K562 lysate. Recommended for use at 2-4ug/ml. Staining (3ug/ml) of K562 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
