Uncoupling Protein 2 (UCP2, SLC25A8, UCPH), Rabbit

Produktübersicht

• Artikelname: Uncoupling Protein 2 (UCP2, SLC25A8, UCPH), Rabbit
• Artikelnummer: USB-U1621-13
• Hersteller Artikelnummer: U1621-13
• Alternativnummer: USB-U1621-13-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Immunogen: Synthetic peptide corresponding to a 13aa sequence between the 3rd and 4th transmembrane domain of human UCP2 conjugated with KLH. Species Sequence Homology: rat and mouse, 100%, canine, bovine and porcine, 92%

Produktbeschreibung

White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7, ~95% homology) mitochondrial uncoupling protein (1). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Western Blot: 1-10ug/ml (ECL) Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue. Reported to detect UCP2 in human neuroblastoma cell shy cells. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: U1621-12: Uncoupling Protein 2, Human, Control Peptide (UCP2, SLC25A8, UCPH) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Reinheit: Purified by immunoaffinity chromatography.
• Formulierung: Supplied as a liquid in PBS, 0.1% BSA.