Recombinant protein corresponding to Leu24-Thr297 from mouse uPAR expressed in NS0 cells
CD87, is a glycosyl phosphatidylinositol-linked receptor which serves to localize and enhance the proteolytic activity of the urokinase-type plasminogen activator (uPA). The uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion and chemotaxis. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Blockade of Receptor-Ligand Interaction: 0.5-2.5ug/ml. Blocks 50% of the binding of 50ng/ml of rhUrokinase to immobilized human rmuPAR (100ul of a 0.5ug/ml solution was coated in each well) in a functional ELISA assay. Western Blot: 1ug/ml using recombinant mouse uPAR Fc Chmiera under non-reducing conditions only Flow Cytometry: 2.5ug/10e6 cells using mouse whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
