Recombinant protein corresponding to Leu23-Arg303 from human PAR, expressed in NSO cells.
CD87, is a glycosyl phosphatidylinositol-linked receptor which serves to localize and enhance the proteolytic activity of the urokinase-type plasminogen activator (uPA). The uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion and chemotaxis. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Blockade of Receptor-Ligand Interaction: ~0.5-1.5ug/ml. Blocks 50% of the binding of 30ng/ml of rhuPAR to immobilized human uPA (100ul of a 0.5ug/ml solution was coated in each well) in a functional ELISA assay. ELISA (capture): 100ul/well of the capture antibody at 4ug/ml Western Blot: 1-2ug/ml. The detection limit is 5ng/lane under non-reducing conditions. Flow Cytometry: 25ug/ml Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml solution of a secondary developing reagent such as IgG (FITC) goat anti-mouse. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
