Urokinase Plasminogen Activator Receptor 1, Fc Chimera, Recombinant, Mouse (uPAR-1/Fc)
Artikelnummer:
USB-U2610-05B
Hersteller Artikelnummer:
U2610-05B
Alternativnummer:
USB-U2610-05B-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
ELISA
Recombinant protein corresponding to mouse uPAR (Leu24-Thr297) at N-terminal and human IgG1 (Pro100 -Lys330) at C-terminal, expressed in NS0 cells. The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR. (Dear, A.E. and R.L. Medcalf, 1998, Eur. J. Biochemistry 252:185-193). A DNA sequence encoding amino acids 1-297 of mouse urokinase-type plasminogen activator receptor one (uPAR-1) (Kristensen, P. et al., 1991, J. Cell Biol. 115:1763-1771) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0. Molecular Mass: Mature recombinant mouse uPAR-1/Fc is a disulfide-linked homodimeric protein. Each monomer of mature mouse uPAR-1/Fc contains 512 amino acid residues and has a calculated molecular mass of 56kD. As a result of glycosylation, the recombinant uPAR-1/Fc migrates as 66-90kD proteins in SDS-PAGE under reducing conditions. Measured by its ability to bind mouse uPA. Activity: Measured by its binding ability in a functional ELISA. Immobilized rmuPAR/Fc Chimera at 5ug/mL (100uL/well) can bind rhuPA with a linear range of 3-200ng/mL. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
