The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100 Optimal working dilutions to be determined by researcher. Hybridoma: SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
