Recombinant protein corresponding to Met1-Lys96 of human VAMP 1 expressed in E. coli.
VAMP-1 (vesicle-associated membrane protein 1, also synaptobrevin-1/SYB1) is an 18kD member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa1-96) and a 22aa transmembrane domain (aa97-118). There is one coiled-coil region between aa33-93. Multiple splice variants are known that show two, three, four and 81aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. over aa1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml, Daudi human Burkitts lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (globus pallidus) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
