Purified, E.coli-derived, recombinant human VAMP-2 (aa1-94, Accession P63027).
VAMP-2 (vesicle associated membrane protein 2, also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116aa in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91) and a membrane-anchor domain (aa95-114). Overaa1-94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
