VRL-1 (Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, Stretch-activated channel 2B), Rabbit

Produktübersicht

• Artikelname: VRL-1 (Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, Stretch-activated channel 2B), Rabbit
• Artikelnummer: USB-V2075-05
• Hersteller Artikelnummer: V2075-05
• Alternativnummer: USB-V2075-05-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Immunogen: Synthetic peptide (18aa) corresponding to the C-terminus of the rat VRL-1 protein.

Produktbeschreibung

Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains. VR1 expression is limited to small to medium diameter primary sensory neurons. Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins, rat 761aa, human 764aa) have been cloned and characterized. There is ~49% identity between VRL-1 and VR1. In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat. Instead, it is activated by high temperatures with a threshold of ~52C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen and intestine) that are unlikely to encounter temperatures of >50C. It is possible that VRL-1 is also activated by stimuli other than heat. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10 ug/ml (ECL). Recombinant VR1 is ~90kD ELISA: 0.5-1ug/ml. V2075-05A Control Peptide can be used to coat ELISA plates at 1ug/ml. Immunohistochemistry: 1-20ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified by immunoaffinity chromatography.
• Formulierung: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.