Applications: Suitable for use in Immunohistochemistry, Radioimmunoassay, and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:300 Immunohistochemistry (frozen): 1:2,000 RIA: 1: 400,000 Western Blot: 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
