Vasoactive intestinal Polypeptide Receptor 2 (VIP Receptor 2, VIP2R, VIPR2, Helodermin Preferring VIP Receptor, Helodermin-preferring VIP Receptor, Pituitary Adenylate Cyclase Activating Polypeptide Type III Receptor, PACAP Type I
Artikelnummer:
USB-V2115-26-HRP
Hersteller Artikelnummer:
V2115-26-HRP
Alternativnummer:
USB-V2115-26-HRP-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
VIPR2 (NP_003373, 24-127aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Vasoactive intestinal peptide (VIP) is a 28a peptide (human, chr 6q26-q27). Expressed and secreted by neurons innervating primary and secondary immune organs such as lymph nodes with a Mol.wt of 20kD. VIP is a potent neurotrophic factor causes vasodilation, lowers arterial blood pressure, and relaxes the smooth muscle of trachea, stomach and gall bladder. VIP also modulates several T-lymphocyte activities including motility, cytokine production, proliferation and apoptosis, VIP exerts its biological activity by binding to two closely related class II G-protein-coupled receptors VPAC-1 and VPAC-2 beside this VIP has its own receptors VIPR1&2 (Vasoactive Intestinal Polypeptide Receptor 1&2) and VIPRRP (VIP receptor-gene repressor protein). VIP shows similarities to glucagon, secretin and gastric inhibitory peptide (GIP) as such it has been considered a member of the glucagon-secretin family. The VIP is 100% conserved in mouse, rat and human. VIP is considered to be a viable candidate for the development of treatments for rheumatoid arthritis, since treatment with VIP significantly reduced incidence of severity of arthritis, the therapeutic effect of VIP was associated with downregulation of both inflammatory and autoimmune components of the disease. VIPR2, a 438aa (chr.7q36.3) protein in human (437aa in rat and mouse). The VIP receptor2 is also termed as type III PACAP receptor, mainly expressed in neural tissues. This is a receptor for VIP as well as PACAP 38 and 27, G proteins mediate the activity of this receptor, which activates adenyl cyclase and can be coupled to phospholipase C. It shows 86% sequence homology with rat VIPR2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
