| Vasopressin (AVP, Arginine-8-Vasopressin), the antidiuretic hormone is cyclic nonapeptide invovled in the homeostasis of body fluid osmolality, blood volume, vascular tone, and blood pressure. Specific actions of AVP include inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenesis, and modulation of ACTH release from pituitary. AVP belongs to the family of vasocative and mitogenic peptide invovled in normal and pathological cell growth and differentiation. AVP exerts its action through binding to specific membrane receptors coupled to distinct second messengers. There are 3 types of AVP receptors: V1a, V1b, and V2 subtypes. The V2 receptor stimulates adenyl cyclase and protein Kinase A, V1 activate phospholipase A2.C, and D, resulting into production of IP3 and DAG, the mobilization of intracellular calcium, the influx of extracellular calcium, the activation of protein Kinase C, and protein phosphorylation. The V1a receptors mediate vasoconstriction and hepatic gluconeogenesis platelet aggregation, coagulation factor release. V1a receptors are found in vascular smooth muscle, hepatocytes, blood platelets, lymphocytes and monocyte, type II pneumocytes, adrenal cortex, brain, reproductive organs, retinal epithelium, renal mesangial cells. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-10ug/ml (ECL). ELISA: 0.5-1ug/ml using 1ug/ml control peptide/well. Immunohistochemistry: 2-10ug in paraformaldehyde fixed sections of tissues Optimal dilutions to be determined by the researcher. Recommended Control Peptide: V2120-20A: Vasopressin (Arg) Receptor V1b, Rat, Control Peptide (AVR-V1b) Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
