A 12 amino acid synthetic peptide sequence from rat VMAT1 (1). This peptide is predicted to be cytoplasmic. Epitope: C-terminal.
VMAT1 has been localized in adrenal chromaffin cells, endocrine and paracrine cells associated with the intestine, stomach and sympathetic nervous system (2,3). VMAT1 has also been found in endosomes of transfected CHO cells and in LDCVs of rat PC12 cells (2). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml (Chemiluminescence technique). Detects a major band at ~55kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells. Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors. Immunohistochemistry: 1-20ug/ml on paraformaldehyde fixed sections. ELISA: 0.5-1.0ug/ml (1ug/ml VMAT1 peptide/well) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
