Purified Vicia villosa lectin (VVA), TRITC conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine TRITC/Protein Ratio (OD550/OD280): 1.28 Activity: More than 250ug/ml is usually required to agglutinate type A1 cells. Less than 1ug/ml will agglutinate neuraminidase treated cells regardless of blood type. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. TRITC conjugates are sensitive to light.
Formulierung:
Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide. Labeled with TRITC.
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