Vimentin (57kD) is the intermediate filament protein (IFP) of mesenchymal cells. It is the only IFP, which deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in rhabdomyosarcomas and leiomyosarcomas. Applications: Suitable for use in Immunoblot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100 Immunohistochemistry (frozen sections): 1:50-1:100. Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Immunoblot: 1:50-1:500 Optimum dilutions to be determined by researcher. Hybridoma: SP2/0-Ag14 mouse myeloma with spleen cells from a BALB/c mouse immunized with cytoskeletal vimentin extract of calf lens. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[4A85]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
