| Virginiamycin complex is defined as a mixture of 75% virginamycin M1 (ostreogrycin A) and 25% virginiamycin S1 and the less abundant S analogs. As the two major components have quite different solubilities these proportions are not readily achieved or used. The individual components are isolated and re-combined to provide the defined components of virginiamycin complex. The structure composition of the complex is important as Virginiamycin S1 acts a synergist, binding to the conformational change of the peptidyl transferase center of the 50S ribosome induced by ostreogrycin A. Synonyms: Cebin V, Eskalin V, Eskamicin, Stafac, Stephylomycin, Mikamycin, Ostreogrycin, Patricin, Pristinamycin, Streptogramin, Vernamycin, N-((6R,9S,10R,13S,15aS,22S,24aS)-22-benzyl-6-ethyl-10,23-dimethyl-5,8,12,15,17,21,24-heptaoxo-13-phenyldocosahydro-12H-pyrido[2,1-f]pyrrolo[2,1-l][1]oxa[4,7,10,13,16]pentaazacyclononadecin-9-yl)-3-hydroxypicolinamide compound with (12Z,6R,7R,8E,13Z,15E,17S)-17-hydroxy-6-isopropyl-7,15-dimethyl-32,33-dihydro-31H-5-oxa-11-aza-1(4,2)-oxazola-3(1,5)-pyrrolacycloicosaphane-8,13,15-triene-2,4,10,19-tetraone (1:1) CAS No: 11006-76-1 Molecular Formula: C28H35N3O7 (Virginiamycin M1) & C43H49N7O10 (Virginiamycin S1) Molecular Weight: 525.6 (Virginiamycin M1) and 823.9 (Virginiamycin S1) Purity: 95% by HPLC (75% Virginamycin M1, 20% Virginiamycin S1, ~1% other minor analogs) Appearance: White solid Solubility: Soluble in DMF or DMSO. Viriginiamycin M1 has only limited solubility in ethanol and methanol. Poor water solubility. Storage and Stability: Lyophilized powder may be stored at -20C and is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
