Vitamin D Receptor (VDR, vitamin D (1,25- dihydroxyvitamin D3) receptor , HGNC:12679, NR1I1, vitamin D (1,25-dihydroxyvitamin D3) receptor), Goat
Artikelnummer:
USB-V2130-03
Hersteller Artikelnummer:
V2130-03
Alternativnummer:
USB-V2130-03-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to CGNQDYKYRVSD of an internal region of human Vitamin D Receptor (NP_000367). Species sequence homology: canine
VDR is a member of the superfamily of steroid hormone receptors. It is known to play a role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation. In avians, VDT appears as a doublet of 58kD and 60kD. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16000. Western Blot: 0.3-1ug/ml. human brain lysates, and mouse and rat small intestines. Optimal dilutions to be determined by the researcher. Control Peptide: V2130-03-P Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See V2130-03-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop.
