Vitronectin (Vn) is a plasma and serum glycoprotein with a normal concentration ranging from 200-400ug/ml. It exists in both a 75kD single-chain form and a 65+10kD two-chain form. Vn can exist in at least two different conformational forms. The majority of Vn found in the circulation is present in the native (''closed) form. In this form, most of the binding sites for other ligands are cryptic. The second form of Vn, the denatured (''open, multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation. This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10kD light chain to the 65kD heavy chain of the two chain form. The liver is the primary site of vitronectin synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages. Vn plays an important role in a number of physiological and pathophysiological processes. It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis. Vn also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Rz Ratio (Reinheitszahl A403/A280): 0.63 as determined spectrophotometrically. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Reinheit:
Purified by affinity chromatography.
Formulierung:
Supplied as a liquid in buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with Horseradish peroxidase (HRP).
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