VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3), IgG1, Clone: [8H125], Mouse, Monoclonal

Produktübersicht

• Artikelname: VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3), IgG1, Clone: [8H125], Mouse, Monoclonal
• Artikelnummer: USB-V2210-02
• Hersteller Artikelnummer: V2210-02
• Alternativnummer: USB-V2210-02-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IHC
• Immunogen: Recombinant protein corresponding to Ser2-Pro185 from human VHR, expressed in E. coli (NM_004090).

Produktbeschreibung

Vaccinia Virus VH1-related phosphatase (VHR), also known as dual-specificity phosphatase 3 (DUSP3), dephosphorylates a variety of substrates at serine, threonine, and tyrosine residues. VHR has been shown to dephosphorylate the mitogen-activated protein kinases ERK1, ERK2, and JNK, but not p38. Phosphorylation of VHR at Y138 by ZAP70 enhances the inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor signaling. Applications: Suitable for use in Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 25ug/mL to detect VHR in paraffin-embedded human tonsil tissue sections. ELISA (Direct): 0.5-1ug/ml.The detection limit for rhVHR is ~1ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [8H125]
• Isotyp: IgG1
• NCBI: 004090
• UniProt: P51452
• Reinheit: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Formulierung: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.