VWA1 (von Willebrand Factor A Domain-containing Protein 1, WARP), IgG1, Clone: [10K63], Mouse, Monoclonal
Artikelnummer:
USB-V2700-15
Hersteller Artikelnummer:
V2700-15
Alternativnummer:
USB-V2700-15-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Recombinant protein corresponding to Arg19-Pro445 from human WARP, expressed in NS0 cells.
Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50kD glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa34-213), two fibronectin type III domains (aa211-301 and 331-421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP is 72% aa identical to mature mouse and rat WARP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, under reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
