Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers. The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): requires protein digestion pre-treatment of paraffin sections. e.g. trypsin or pronase. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
