WAF1, p21 (Wild-type p53-activated Fragment, Cip1, Sdi1, Pic1 Tumor Suppressor Protein) (MaxLight 490), Clone: [0.T.180/0.T.180A], Mouse, Monoclonal
Artikelnummer:
USB-W0750-05-ML490
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| Artikelname: | WAF1, p21 (Wild-type p53-activated Fragment, Cip1, Sdi1, Pic1 Tumor Suppressor Protein) (MaxLight 490), Clone: [0.T.180/0.T.180A], Mouse, Monoclonal |
| Artikelnummer: | USB-W0750-05-ML490 |
| Hersteller Artikelnummer: | W0750-05-ML490 |
| Alternativnummer: | USB-W0750-05-ML490-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IP, WB |
| Immunogen: | Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag. |
| MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies. Pooled monoclonal mouse IgGs Application(s): Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4ug immunoprecipitates Cip1 from 500ug of HeLa nuclear extract. Immunoblot Analysis: 0.5-2ug/ml detects WAF1, p21 in 20ug of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Control: HeLa nuclear extract. Use 20ug per lane for minigels. Note: Applications are based on unconjugated antibody. |
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