WFDC2 (WAP Four-disulfide Core Domain Protein 2, Epididymal Secretory Protein E4, Major Epididymis-specific Protein E4, Putative Protease Inhibitor WAP5, HE4, WAP5), Sheep
Artikelnummer:
USB-W1023-45
Hersteller Artikelnummer:
W1023-45
Alternativnummer:
USB-W1023-45-100
Hersteller:
US Biological
Wirt:
Sheep
Kategorie:
Antikörper
Applikation:
ELISA, IP, WB
Immunogen:
Purified, NS0-derived, recombinant human HE4 isoform 1 (Thr28-Phe124, Accession Q14508).
HE4 (also WDFC2, WAP Four-disulfide core domain protein 2) is a secreted, 25kD glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94aa in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa31-73 and 74-123). There are four potential splice variants. One shows a deletion of aa27-74, while three others show aa substitutions: 28aa for aa75-124, 23aa for aa1-74, and 10aa for aa71-124. Over aa31-124, human HE4 shares 63% aa identity with an extended (144 aa) mouse form of mature HE4. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml ELISA (direct): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
