WFDC2 (WAP Four-disulfide Core Domain Protein 2, Epididymal Secretory Protein E4, Major Epididymis-specific Protein E4, Putative Protease Inhibitor WAP5, HE4, WAP5), Goat
Artikelnummer:
USB-W1023-45A
Hersteller Artikelnummer:
W1023-45A
Alternativnummer:
USB-W1023-45A-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to GAEKTGVCPELQADQ, from human WFDC2, at the internal region of the protein (NP_006094.3).
HE4 (also WDFC2, WAP Four-disulfide core domain protein 2) is a secreted, 25kD glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94aa in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa31-73 and 74-123). There are four potential splice variants. One shows a deletion of aa27-74, while three others show aa substitutions: 28aa for aa75-124, 23aa for aa1-74, and 10aa for aa71-124. Over aa31-124, human HE4 shares 63% aa identity with an extended (144aa) mouse form of mature HE4. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.2-1ug/ml, transfected HEK293 transiently expressing full-length Human WFDC2 (myc and DYKDDDDK tagged) on ~25kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
