| The human WSTF gene is located within the common Williams Syndrome (WS) deletion area at chromosome 7q11.23. Several WSTF gene products have been detected with little difference in length of polypeptides (1-3). Functional motifs identified by sequence-homology searches include a PHD-type zinc finger motif followed by a bromodomain. Both motifs are found in many transcriptional factors, suggesting that WSTF may function as a transcription factor. A Drosophila gene (acf1) was cloned, which encodes two forms of Acf1 proteins with molecular weight 170kD and 185kD, respectively (4). It was demonstrated that Acf1 is structurally related with the human WSTF gene. Acf1 forms a complex with another protein, ISWI, and functions in the ATP-dependent catalysis of chromatin assembly (4). Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
