WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1), Rabbit

Produktübersicht

• Artikelname: WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1), Rabbit
• Artikelnummer: USB-W2996-01
• Hersteller Artikelnummer: W2996-01
• Alternativnummer: USB-W2996-01-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: A 17-aa peptide sequence, mapping near the N-terminus and prior to the tyrosine kinase catalytic domain of human WNK-1, conjugated KLH. Species Sequence Homology: monkey, chimp, mouse, rat and guinea pig

Produktbeschreibung

Protein kinases represent a superfamily of over 400 members that share a catalytic domain of 250-300 amino acids. A conserved lysine, in subdomain II of the catalytic core of all 390 protein kinases, anchors a and b phosphoryl groups of ATP. The catalytic domain of WNK (With No K=Lysine) kinases contains all invariant residues, except for this lysine residue. There are 4 (identified) WNK genes (WNK1, WNK2, WNK3 and WNK4) in human (h) and rat (r). The hWNK kinases exhibit ~30% seq identity and 50% seq homology to human Mitogen Activated Protein kinases (MAPK) STK2, PAK2, MEKK3, PAK3 and Raf-1. WNK4 (human 1243-aa, 17q21) is a Ser-Thr kinase in which the conserved catalytic lysine is replaced by a cysteine but another lysine residue in subdomain I confers the kinase activity. WNK4 is 76% identical to WNK1 in kinase domain. WNK4 is expressed predominantly in kidney, colon and skin. WNK4 localize to the distal convoluted tubule (DCT) and cortical collecting duct (CCD), that play a key role in salt, water, K+ and pH homeostasis. WNK4 is present exclusively in the intracellular tight junctions in DCT and in both cytoplasm and tight junctions in CCD. In DCT, salt reabsorption is mediated by the electroneutral Na-Cl cotransporter. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000. Control peptide can be used to coat ELISA plates at 50-100ng control peptide/well Western Blot: 1:1000-5000 (Chemiluminescence) Optimal dilutions to be determined by the researcher. Control Peptide: W2996: WNK1, Human, Control Peptide (With-No-Lysine Kinase 1) Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified by immunoaffinity chromatography
• Formulierung: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.