XbaI

Produktübersicht

• Artikelname: XbaI
• Artikelnummer: USB-X0989
• Hersteller Artikelnummer: X0989
• Alternativnummer: USB-X0989-3000,USB-X0989-5X1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Description: 5-T C T A G A-3 3-A G A T C T-5 Source: Xanthomonas badrii Supplied With: R1625: Restriction Enzyme Buffer A, 10X Concentration: 10u/ul Storage Buffer: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 7mM 2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Unit Definition: One unit is defined as the amount of XbaI required to digest 1ug of lambda DNA-dam-SmaI fragments in 1 hour at 37C in 50ul of assay buffer. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Quality Control Assay Data: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hr). Ligation/Recutting Assay: The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of XbaI for 4 hr. Blue/White Cloning Assay: The B/W assay was replaced with LO test after validating experiments showed LO test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Enzyme Properties: Methylation Effects: Dam: may overlap - blocked Dcm, CpG, EcoKl, EcoBl: never overlaps- no effect Stability during Prolonged Incubation: A minimum of 0.1 units of XbaI is required for complete digestion of 1ug of lambda DNA in 16 hr at 37C. Digestion of Agarose-embedded DNA: A minimum of 5 units of XbaI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hr. Compatible Ends: BcuI, Eco130I, NheI, XmaJI Number of Recognition Sites in DNA: Lambda: 1 PhiX174: 0 pBR322: 0 pUC57: 1 pUC18/19: 1 pTZ19R/U: 1 M13mp18/19: 1 Thermal Inactivation: XbaI is inactivated by incubation at 65C for 20min Storage and Stability: For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.