XhoI

Produktübersicht

• Artikelname: XhoI
• Artikelnummer: USB-X1030
• Hersteller Artikelnummer: X1030
• Alternativnummer: USB-X1030-2000,USB-X1030-5000,USB-X1030-5X2000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-CT C G A G-3 3-G A G C TC-5 Form: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. R1625 (double digests): Supplied as a liquid in 33mM Tris-acetate, (pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37C R1625-04 (for 100% digestion): Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C. Unit Definition: One unit is defined as the amount of XhoI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Protocol for Digestion: Add: Nuclease free water: 16ul R1625-04: 2ul DNA (0.5-1ug/ml): 1ul X1030: 0.5-2ul Mix gently and spin down for a few seconds. Incubate at 37C for 1-16 hours. Protocol for Digestion Directly after Amplification: Add: PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA) Nuclease free water: 18ul R1625-04: 2ul X1030: 1-2ul Mix gently and spin down for a few seconds. Incubate at 37C for 1-16 hours. Stability during Prolonged Incubation: A minimum of 0.1 units of XhoI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: XhoI is inactivated by incubation at 80C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of XhoI is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: BauI, Eco88I, SalI, SmlI Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with NdeI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Xhol, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.004uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Xhol for 4 hours. Blue/White Cloning Assay: Replaced by Labeled Oligonucleotide (LO) Assay Compatible Ends: Eco88I, PspXI, SalI, SmoI, SgrDI. Number of Recognition Sites in DNA: Lambda, PhiX174: 1 M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U: 0 Methylation Effects: MbiI, EcoBl, Dam, Dcm, EcoKll never overlaps CpG completely cleavage impaired Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.