XmaJI (AvrII)

Produktübersicht

• Artikelname: XmaJI (AvrII)
• Artikelnummer: USB-X1043
• Hersteller Artikelnummer: X1043
• Alternativnummer: USB-X1043-200,USB-X1043-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Sequence: 5-CC T A G G-3 3-G G A T CC-5 Source: Xanthomonas maltophilia Jo 85-025 Concentration: 10 units/ul Unit Definition: One unit is defined as the amount of XmaJI required to digest 1ug of lambda DNA-Smal fragments in 1 hour at 37C in 50ul of reaction buffer. Supplied with: R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent and Storage Buffer: 10mM Tris-HCl , pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hour) with XmaJI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with XmaJI more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.06uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10 units of XmaJI for 4 hours. Recognition Sites: Lambda: 2 PhiX174: 0 pBR322: 0 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10 units of XmaJI for 16 hours. After religation and transformation <1% of white colonies were detected. Stability during Prolonged Incubation: A minimum of 0.2 units of XmaJI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: XmaJI is inactivated by incubation at 80C for 20 minutes. Digestion of Agarose-embedded DNA: A minimum of 10 units of the enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: BcuI, NheI, XbaI, Eco130I (CCTAGG) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.