E. coli-derived recombinant protein corresponding to Met1-Gln622 from human APP1.
Aminopeptidase P1 (APP1), also known as XPNPEP1 (X-prolyl aminopeptidase), is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. Deficiency of X-prolyl aminopeptidase results in excretion of large amounts of imino-oligopeptides in urine. The sequence of human APP1 is 99%, 97%, and 95% identical to that of canine, bovine, and mouse/rat. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with recombinant human Aminopeptidase P1/XPNPEP1 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
