| Y-27632 (also called Y27632 or Y 27632) is a potent, cell permeable, selective and ATP-competitive Rho-associated protein kinases inhibitor, including p160ROCK, ROCK-II and PRK2 inhibitor. Ki values are 0.14, 26, 25 and >250uM for p160ROCK, PKC, cAMP-dependent protein kinase and myosin light-chain kinase, respectively. Prevents apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem cells (hES) without affecting their pluripotency. Tumor cell invasion and metastasis suppressor. Smooth muscle relaxant. Decreases liver fibrosis by hepatic stellate cell growth inhibition. Antinociceptive. Blocks generation of inflammatory cytokines. Stem cell research modulator. Increases survival rate of hES cells undergoing cryopreservation. Synonyms: (R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride, trans-4-[(R)-1-[(tert-Butyloxycarbonyl)amino]ethyl]-N-(4-pyridyl)cyclohexanecarboxamide-d4, [(1R)-1-[trans-4-[(4-Pyridinyl-d4-amino)carbonyl]cyclohexyl]ethyl]-carbamic Acid 1,1-Dimethyethyl Ester CAS No: 146986-50-7 Molecular Formula: C14H21N3O2HCl Molecular Weight: 351.48 Purity: 98% (HPLC). Contains approx.1.4% water. Appearance: Supplied as a white to off-white solid. Melting Point: 180-230C (lit) Solubility: Water (30mg/ml), DMSO (25mg/ml), Methanol (25mg/ml) Method of Determining Identity: 1H-NMR Note: A Pfizer Compound Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive |
