Recombinant protein corresponding to human YAP protein expressed in bacteria. Cellular Localization: Nuclear.
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. As one of several genes of 1q22 amplicon, YAP was recently implicated in liver and breast cancer. Overexpression of YAP gene in MCF10A mammary cell line induces oncogenic transformation. Applications: Suitable for use in ChIP, Immunohistochemistry, Immunoprecipitation, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 2-10ug/ml Immunohistochemistry (Frozen/Paraffin): 1:50-1:200 Western Blot: 1:1000. Detects 65kD band using transfected HEK 293 lysate as sample. Immunoprecipitation: 2-10ug Optimal dilutions to be determined by the researcher. Positive Control: Brain and kidney lysates and HEK 293 cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
