Synthetic peptide corresponding to C-TYHSRDESTDS, from the internal region of human YAP1 (NP_006097.1).
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:8000 Western Blot: 1-3ug/ml ~90kD band observed in lysates of cell line HepG2 (calculated MW of 48.8kD according to NP_006097.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.