| ZAG (zinc-a2-glycoprotein, also ZA2G) is a 40kD, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26-201) and a C2-type Ig-like domain (aa 207-292). Two alternate splice forms exist, one shows a 66 aa substitution for the C-terminal 30 aa, and a second shows a nine Lys substitution for aa 151-298. Mature human ZAG is 60% aa identical to mouse ZAG. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5 -1.0ug/ml detection limit for rhZAG is ~ 0.3ng/well. Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
