ZAP 70 (TCR Zeta Associated Protein Kinase 70kD), Goat
Artikelnummer:
USB-Z0300-01F
Hersteller Artikelnummer:
Z0300-01F
Alternativnummer:
USB-Z0300-01F-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
E. coli-derived recombinant human ZAP70. (aa 163 - 254)
ZAP70 (zeta-chain (TCR) associated protein kinase 70kD), expressed primarily in T and NK cells, is a Syk family cytosolic protein tyrosine kinase that consists of two N-terminal SH2 domains and a C-terminal tyrosine kinase domain. Upon T cell receptor activation and phosphorylation of TCR ITAMs by Src family kinases, ZAP70 is recruited to phosphorylated ITAM sequences and subsequently phosphorylated on several tyrosine residues. ZAP70 has been implicated in several immune disorders. An autosomal recessive form of SCID in humans has been attributed to a homozygous mutation in the kinase domain of ZAP70. ZAP70 expression also defines an aggressive subset of CLL. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
