Peptide derived from a.a. 326-341 from the central portion of the human ZAP-70 protein sequence just upstream of the protein kinase domain. Shares 87.5% homology with the mouse ZAP-70 peptide.
Engagement of the T-cell receptor (TCR) by antigen activates a biochemical cascade that involves both protein tyrosine kinases (PTKs) and tyrosine phosphatases and culminates in cytokine release, cellular proliferation, and cellular differentiation. Although the multi-chain TCR does not possess intrinsic kinase activity, at least three PTKs have been implicated in proximal TCR signaling events including: Fyn, Lck, and ZAP70. Fyn and Lck are members of the Src-family of tyrosine kinases, whereas ZAP70 is a member of the Syk family. ZAP70 is a 70kD PTK and can be functionally divided into three domains: two SH2 domains, and a C-terminal PTK domain. As described, ZAP70 is structurally homologous to the Syk PTK. However, ZAP70 and Syk have distinct tissue distributions with ZAP70 expressed exclusively in T-cells and natural killer cells, and Syk expressed predominantly in B cells, thymocytes, and myleoid cells. Although these two PTKs have different tissue distributions, both play essential roles in the signal transduction pathways of various hematopoetic antigen receptors. In response to engagement of the TCR, ZAP70 undergoes tyrosine phosphorylation and associates with the tyrosine phosphorylated TCRz and CD3 subunits. Tyrosine phosphorylation of TCRz and ZAP70, and their subsequent association is dependent upon the Lck and Fyn PTKs. Association of ZAP70 with the TCR requires receptor stimulation and is mediated by two phosphorylated tyrosine residues present in a 17 amino acid motif referred to as ITAM for intracellular tyrosine activation motif. This motif is present in the TCRz chain, as well as In the CD3-e,-g,-d subunits. The essential role for ZAP70 in T-cell development and activation has been perhaps best demonstrated by studies showing a loss of T cell signaling when the recruitment of ZAP70 to the activated TCR is disrupted, and by the identification of patients with severe combined immunodeficiency who carry a mutation of ZAP70 which results in the production of a kinase defective protein. Recent evidence has suggested that ZAP70 should be considered as an effective biomarker in Chronic Lymphocytic Leukemia (CLL). Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.1-1.0ug/ml Western Blot: 1ug/ml Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher. Positive Control: Human Jurkat cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3G301]
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
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