A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.
ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as motif-a. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions:. Immunohistochemistry: Frozen sections Immunohistochemistry: For formalin fixed, paraffin embedded tissue sections. Protease XIV pretreatment is required Optimal dilutions to be determined by the researcher. Positive Controls for Immunoprecipitation: Canine MDCK and human Caco-2 cells. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish peroxidase (HRP).
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