Synthetic peptide corresponding to the central portion of the canine ZO-2 protein.
MaxLight(TM) 490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor supressor gene product (dlg-A) of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90. These proteins share several conserved domains and are collectively referred to as the MAGUK family. The regions of homology among this family of proteins include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated domains termed PDZ domains. The ZO-1 and ZO-2 proteins are distinct from the other family member in that they possess C-terminal acidic and proline rich regions, and alternatively spliced domains at their C-termini. Although, ZO-1 and ZO-2 are structurally similar and display significant sequence homology, the proteins are divergent at their C-termini. This suggests that the C-terminal ends of ZO-1 and ZO-2 have distinct functions. The canine ZO-2 protein has a predicted molecular mass of ~132kD. However, the protein migrates with a mass of 160kD when analyzed by SDS-PAGE. Applications: Suitable for use in FLISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: FLISA: 0.1-1ug/ml Western Blot: 1-4ug/ml Immunohistochemistry (Mouse liver tissues): 4-5ug/ml Note: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining. Optimal dilutions to be determined by the researcher. Positive Control: HeLa and canine MDCK cell lysates and mouse liver homogenates. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight(TM)490.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
