Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg

Artikelnummer: VEC-B-1045
Artikelname: Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg
Artikelnummer: VEC-B-1045
Hersteller Artikelnummer: B-1045
Alternativnummer: VEC-B-1045
Hersteller: Vector Laboratories
Kategorie: Sonstiges
Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg

Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity.  By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Biotinylated Lens culinaris agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

 

Specifications

 
Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide, 10 mM methyl-alpha-D-mannopyranoside.
Concentration 5 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Mannose, Glucose