The biotinylated goat anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Recognizes both heavy and light chains (H+L)
Cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is at the same concentration as that provided in the VECTASTAIN® ABC kits.
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