FLUORESCEIN WHEAT GERM AGGLUTININ

Artikelnummer: VEC-FL-1021
Artikelname: FLUORESCEIN WHEAT GERM AGGLUTININ
Artikelnummer: VEC-FL-1021
Hersteller Artikelnummer: FL-1021
Alternativnummer: VEC-FL-1021-5, FL-1021-10, FL-1021-5, FL-1021-10
Hersteller: Vector Laboratories
Kategorie: Proteine/Peptide
Spezies Reaktivität: Plant
Konjugation: FITC

Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

More Information
Unit Size 5 mg, 10 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine