Your path to simplified multiplexing starts with VectaPlex™.
The VectaPlex Antibody Removal kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA) workflows.
VectaPlex simplifies sample processing for manual workflows, accelerates multiplex assay optimization, preserves precious sample, and maintains tissue integrity and morphology through multiple cycles of antibody binding and removal.
Benefits include:
• Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections
• Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows
• Obtain more spatial biomarker information from each tissue section
• Stain with multiple primaries from the same species and a single secondary without cross reactivity
• Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
• Maintains tissue antigenicity through multiple cycles
• Primary antibody concentrations do not need to be optimized based on the order of detection in multiplex workflows
• Easy-to-use, room temperature application
Specifications
Unit Size
1 kit
Applications
Immunofluorescence, Tyramide Signal Amplification
Recommended Usage
(15–25° C)
Target Species
Universal
Format
Ready-to-Use
Kit Contents
VectaPlex™ Antibody Removal Reagent A
VectaPlex™ Antibody Removal Reagent B
Formulierung:
Ready-to-Use
Lagerung:
15–25° C
Target-Kategorie:
Universal
Image captured after completion of 3 rounds of tyramide staining.
Vectaplex™ was used after each round. FFPE tonisl; 3 mouse primary antibodies (CD3 (Red), CD68 (Green), AE1/AE3 (Purple)); detected using Opal 3-Plex TSA Kit.
Composite image generated from individual images taken during 6 rounds of IF staining using Vectaplex™. FFPE stomach; 6 mouse primaries (CD20(Red), CD34 (Yellow), DES (Cyan), AE1/AE3 (Magenta), CD3 (Grey), VIM (Green)); DyLight™ 488 horse anti-mouse lgG.
Vectaplex™ Antibody Removal Kit
VectaPlex™ Antibody Removal Kit
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