ACE2 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ACE2 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX101395
• Supplier Catalog Number: GTX101395
• Alternative Catalog Number: GTX101395-100,GTX101395-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FACS, ICC, IHC-P, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: angiotensin I converting enzyme 2 , ACEH

Product Properties

• Clonality: Polyclonal
• Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 92
• NCBI: 59272
• UniProt: Q9BYF1
• Buffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min