Western Blot Troubleshooting Tips A comprehensive selection of troubleshooting tips

Our partner GeneTex supports researchers with a comprehensive selection of troubleshooting tips for many issues which can occur in a western blot (WB) experiment. Download also the

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Protein Isolation How to Choose Membrane Protein Isolation Kits

Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes.  Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins, carriers, voltage-gated ion channels, CD antigens and many drug targets. The detec...

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Artifacts in IHC: the usual suspects - part I

    After a few intense days of meticulous tissue washes and antibody incubations my staining is finally ready. I am staring at it down the microscope. I enjoy what I see: a dark brown color exactly where I expect to see it! Am I good or what? I give myself a figurative pat on the back and an imaginary high five. Excited and proud, I call my supervisor who glares intently at it and t...

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Calibration and Performance Tracking of Flow Cytometer SpheroTechnical Notes #8

The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run. These part...

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In vivo magnetofection How to use OZ Biosciences' in vivo magnetofection™

The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo mi...

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IHC Protocol using Proteinase K based Antigen Retrieval Immunohistochemistry protocol for formalin-fixed, paraffin embedded ti...

Tissue Sectioning, Deparaffinization, and Rehydration Section paraffin blocks into 4 micron sections with microtome and place on charged microscope slides (Fisher, ProbeOn, Cat. #22230900). Heat slides in a tissue-drying oven for 45 minutes at 60°C. Wash slides in 3 changes of xylene for 5 minutes each at room temperature. Wash slides in 3 changes of 100% alcohol for 3 minutes each at room temperature. Wash slides in 2 changes...

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Standard IHC Protocol Atlas Antibodies standard immunohistochemistry protocol

        Described is the Atlas Antibodies standard immunohistochemistry protocol optimized for Triple A Polyclonals and PrecisA Monoclonals.   Deparaffinization Paraffin sections of 4 µm thickness are baked overnight at 50°C. Prior to immunostaining, depa...

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