Western Blot A comprehensive selection of troubleshooting tips
Our partner GeneTex supports researchers with a comprehensive selection of troubleshooting tips for many issues which can occur in a western blot (WB) experiment. Download also the
Protein Isolation How to Choose Membrane Protein Isolation Kits
Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes. Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins, carriers, voltage-gated ion channels, CD antigens and many drug targ...
Artifacts in IHC The usual suspects - part I
After a few intense days of meticulous tissue washes and antibody incubations my staining is finally ready. I am staring at it down the microscope. I enjoy what I see: a dark brown color exactly where I expect to see it! Am I good or what? I give myself a figurative pat on the back and an imaginary high five. Excited and proud, I call my supervisor who glares intently at it and...
Flow Cytometry Calibration and Performance Tracking - SpheroTechnical Notes #8
The SPHERO™ Calibration Particles are versatile, stable, economical and convenient to use. These particles contain a mixture of fluorochrome which are spectrally similar to many of the fluorochromes used in flow cytometry. As a result, they are used for routine alignment, day-to-day performance verification and long term performance tracking of several channels of flow cytometers in one run. These part...
In vivo magnetofection How to use OZ Biosciences' in vivo magnetofection™
The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo mi...
IHC Protocol using Proteinase K based Antigen Retrieval Immunohistochemistry protocol for formalin-fixed, paraffin embedded ti...
Tissue Sectioning, Deparaffinization, and Rehydration Section paraffin blocks into 4 micron sections with microtome and place on charged microscope slides (Fisher, ProbeOn, Cat. #22230900). Heat slides in a tissue-drying oven for 45 minutes at 60°C. Wash slides in 3 changes of xylene for 5 minutes each at room temperature. Wash slides in 3 changes of 100% alcohol for 3 minutes each at room temperature. Wash slides in 2 changes...
Standard IHC Protocol Atlas Antibodies standard immunohistochemistry protocol
Described is the Atlas Antibodies standard immunohistochemistry protocol optimized for Triple A Polyclonals and PrecisA Monoclonals. Deparaffinization Paraffin sections of 4 µm thickness are baked overnight at 50°C. Prior to immunostaining, depa...