SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257], Unconjugated, Rabbit, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257], Unconjugated, Rabbit, Monoclonal
• Biozol Catalog Number: GTX635692
• Supplier Catalog Number: GTX635692
• Alternative Catalog Number: GTX635692-100,GTX635692-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IHC-P, WB
• Species Reactivity: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [HL257]
• Sensitivity: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.
• Buffer: PBS, no preservative.
• Purity: Affinity purified by Protein A.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) protein (GTX01546-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) and SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (GTX136085-pro) (0.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 Spike S1 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), Brazil variant, His tag (0.25 μg, GTX136094-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 Spike S1 (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), South Africa variant, His tag (0.25 μg, GTX136095-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded infected lung tissue derived from the AdV-hACE2-transduced BALB/c mice model.
SARS-CoV-2 (COVID-19) Spike RBD stained by IgG control (left) and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000 (right).
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human placenta.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human lung.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.