BasoFlowEx Kit

Catalog Number: EXB-ED7043
Article Name: BasoFlowEx Kit
Biozol Catalog Number: EXB-ED7043
Supplier Catalog Number: ED7043
Alternative Catalog Number: EXB-ED7043
Manufacturer: EXBIO
Category: Kits/Assays
Application: FC
The BasoFlowEx Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation. Commercially available allergens can be

 

The BasoFlowEx Kit (CE IVD) is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation.
Commercially available allergens can be used for the stimulation.

The product is intended for In Vitro Diagnostic Use. 

 

Application

Flow Cytometry

Reactivity

Human

Content

ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
ED7043-4 Lysing Solution – 30 ml.

Storage: 2°C to 8°C
Application Notes: FC (QC tested)
Content ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes. ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls. ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled. ED7043-4 Lysing Solution – 30 ml.
Flow cytometric analysis of BasoFlowEx Kit. (A) Visualization of compensated data on the side-scatter (SSC) versus forward scatter (FSC) in dot-plot. (B) Visualization of single cells gate on the FSC-Area versus FSC-height in dot-plot. (C) Visualization of basophil population (CD203 positive versus CD63 positive in dot-plot). (D) Visualization of stimulated (shown in red) and non-stimulated basophil population (shown in dashed line).