Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves chymase C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in mast cell granules within the cell and is the major secreated mast cell protease. Secreated mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Not recommended for Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry/Immunocytochemistry: 0.5-1ug/ml, For optimal results, tissues or cytospin preparations should be fixed in Carnoys Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. Optimal dilutions to be determined by the researcher. Positive Control: Lung, Heart, Skin, Placenta Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
