This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-beta-actin [1:500] with goat anti-rabbit [1:10,000] p/n 711-1122 and goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. *P<0.05 vs. control. KIF, kinesin family member, shRNA, short hairpin RNA. Figure 2. PMID: 30664160.
KIF20B knockdown inhibits cell proliferation. (D) Expression of proliferation marker PCNA in shRNA cells and control cells, measured by western Blotting. Primary antibodies used mouse anti-(PCNA) proliferating cell nuclear antigen [1:1000] and mouse anti-beta-actin [1:500] with goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. Knocking down KIF20B inhibited PCNA expression. *P<0.05 vs. control. KIF, kinesin family member, PCNA, proliferating cell nuclear antigen, shRNA, short hairpin RNA. Figure 3. PMID: 30664160.
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