Goat Antiserum anti-Mouse IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat Antiserum anti-Mouse IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-110-1103
• Supplier Catalog Number: 110-1103
• Alternative Catalog Number: ROC-110-1103
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: goat anti-Mouse IgG F(c) fragment Antibody, goat anti-Mouse IgG Fc fragment Antibody, rabbit anti Mouse IgG Fc
• Format: Antiserum
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 85 mg/mL by Refractometry
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Images

KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-beta-actin [1:500] with goat anti-rabbit [1:10,000] p/n 711-1122 and goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. *P<0.05 vs. control. KIF, kinesin family member, shRNA, short hairpin RNA. Figure 2. PMID: 30664160.

KIF20B knockdown inhibits cell proliferation. (D) Expression of proliferation marker PCNA in shRNA cells and control cells, measured by western Blotting. Primary antibodies used mouse anti-(PCNA) proliferating cell nuclear antigen [1:1000] and mouse anti-beta-actin [1:500] with goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. Knocking down KIF20B inhibited PCNA expression. *P<0.05 vs. control. KIF, kinesin family member, PCNA, proliferating cell nuclear antigen, shRNA, short hairpin RNA. Figure 3. PMID: 30664160.